髌骨细菌门的遗传操作提供微生物暗物质和附生生活方式的机理启示

髌骨细菌门的遗传操作提供微生物暗物质和附生生活方式的机理启示

作者：小柯机器人 发布时间：2023/9/10 15:56:17

本期文章：《细胞》：Online/在线发表

美国华盛顿大学Joseph D. Mougous研究小组发现，髌骨细菌门的遗传操作提供微生物暗物质和附生生活方式的机理启示。该项研究成果于2023年9月7日在线发表在《细胞》杂志上。

研究人员表示，髌骨细菌门（Patescibacteria），又称候选门级辐射类群(candidate phyla radiation)，是一个种类繁多的细菌群，在微生物暗物质中占有不成比例的巨大比例。其少数培养成员主要属于解糖微小寄生菌（Saccharibacteria），以附生体的形式生长在寄主放线菌上。由于缺乏合适的工具，这种生活方式的遗传学基础以及Patescibacteira的其他独特特征仍有待探索。

研究人员展示了Saccharibacteria的自然能力，并利用这一特性对其进行遗传操作。荧光蛋白标记的Saccharibacteria成像技术提供了伴随附生生长现象的高时空分辨率，转座子插入测序（Tn-seq）全基因组筛选揭示了Saccharibacteria神秘基因对其宿主生长的贡献。最后，研究人员利用元基因组数据提供了基于蛋白质结构的尖端生物信息学资源，支持Southlakia epibionticum及其相应的宿主以色列放线菌（Actinomyces israelii）作为揭示附生生活方式分子基础的模型系统。

附：英文原文

Title: Genetic manipulation of Patescibacteria provides mechanistic insights into microbial dark matter and the epibiotic lifestyle

Author: Yaxi Wang, Larry A. Gallagher, Pia A. Andrade, Andi Liu, Ian R. Humphreys, Serdar Turkarslan, Kevin J. Cutler, Mario L. Arrieta-Ortiz, Yaqiao Li, Matthew C. Radey, Jeffrey S. McLean, Qian Cong, David Baker, Nitin S. Baliga, S. Brook Peterson, Joseph D. Mougous

Issue&Volume:

Abstract: Patescibacteria, also known as the candidate phyla radiation (CPR), are a diverse group of bacteria that constitute a disproportionately large fraction of microbial dark matter. Its few cultivated members, belonging mostly to Saccharibacteria, grow as epibionts on host Actinobacteria. Due to a lack of suitable tools, the genetic basis of this lifestyle and other unique features of Patescibacteira remain unexplored. Here, we show that Saccharibacteria exhibit natural competence, and we exploit this property for their genetic manipulation. Imaging of fluorescent protein-labeled Saccharibacteria provides high spatiotemporal resolution of phenomena accompanying epibiotic growth, and a transposon-insertion sequencing (Tn-seq) genome-wide screen reveals the contribution of enigmatic Saccharibacterial genes to growth on their hosts. Finally, we leverage metagenomic data to provide cutting-edge protein structure-based bioinformatic resources that support the strain Southlakia epibionticum and its corresponding host, Actinomyces israelii, as a model system for unlocking the molecular underpinnings of the epibiotic lifestyle.

DOI: 10.1016/j.cell.2023.08.017

Source: https://www.cell.com/cell/fulltext/S0092-8674(23)00906-6

期刊信息

Cell：《细胞》，创刊于1974年。隶属于细胞出版社，最新IF：66.85

官方网址：https://www.cell.com/

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